BREAKING! Murine Study Reveals Anti-Aging Power of Poly-L-Lactic Acid Microspheres for Skin Rejuvenation
Nikhil Prasad Fact checked by:Thailand Medical News Team Jun 04, 2024 5 months, 2 weeks, 5 days, 19 hours, 2 minutes ago
Thailand Aesthetics: In a groundbreaking study, researchers from Nanfang Hospital at Southern Medical University, Guangdong Second Provincial General Hospital, and the First Affiliated Hospital of Sun Yat-sen University in Guangzhou, China, have discovered that poly-L-lactic acid (PLLA) microspheres can significantly delay the aging of epidermal stem cells (EpiSCs). This exciting development that is covered in this
Thailand Aesthetics news report, could revolutionize the field of facial rejuvenation and anti-aging treatments.
Effects of PLLA microspheres on migration and senescence of epidermal stem cells. (A) Schematic diagram, (B) images and (E) statistical analysis of Transwell migration assay of EpiSCs with different treatments. (C) Schematic diagram and (D) images of wound healing results of EpiSCs in different groups and (F) its statistical results. (G) β-Galactosidase (β-Gal) staining images of different groups. (H) qPCR analysis of mRNA expression levels of p16 and p21 in different treatment groups
Injectable Skin Fillers: A New Frontier in Anti-Aging
Injectable skin fillers have become a popular choice for those seeking to combat the visible signs of aging. Among these, PLLA microspheres have gained favor due to their degradable and long-lasting properties. Previous studies have focused on the effects of PLLA on dermal collagen, but this new research shifts the focus to the epidermis, particularly the impact on EpiSCs.
Objective and Methods
The objective of this study was to explore the effects of PLLA microspheres on EpiSCs. Researchers used various concentrations of PLLA microspheres in vitro to culture and identify primary rat EpiSCs. They employed several techniques, including CCK-8 detection, apoptosis staining, flow cytometry, Transwell assay, wound healing assay, q-PCR analysis, and immunofluorescence staining, to observe the effects of PLLA on EpiSCs. Furthermore, they injected PLLA into the dermis of rat skin in vivo to study its impact on the epidermis.
Results: A Promising Discovery
-Enhanced Cell Proliferation and Migration
The study revealed that PLLA microspheres promote cell proliferation and migration while delaying cell senescence and maintaining the stemness of EpiSCs. In vitro experiments showed that intradermal injection of PLLA microspheres into the back skin of rats resulted in delayed aging, as evidenced by histological and immunohistochemical staining of the skin over follow-up periods of 2, 4, and 12 weeks.
Detailed Findings
-
Release and Degradation of PLLA Microspheres: The study observed the gradual degradation of PLLA microspheres over time, indicating a slow release process. This degradation was faster in the presence of K-SFM compared to PBS, with microspheres exhibiting a
concave morphology over time.
-Characterization of EpiSCs: Isolated rat EpiSCs showed high expression of basal cell membrane-related markers, confirming their identity. Flow cytometry results indicated a high purity level of intergrain-α6 and p63-double positive cells.
-Enhanced Proliferation: PLLA concentrations of 250 μg/mL and 500 μg/mL significantly enhanced cell viability after 48 hours, with no induction of cell death or significant differences in apoptosis rates.
-Delayed Senescence: PLLA delayed the aging of EpiSCs, as indicated by reduced mRNA levels of p16 and p21 and enhanced proliferation and migration abilities in wound healing assays.
-Decreased Differentiation: PLLA maintained stemness and mitigated differentiation of EpiSCs, with lower expression of differentiation markers like keratin 10 and higher expression of integrin-α6 and CK19.
In Vivo Effects of PLLA
In vivo studies involved intradermal injection of PLLA microspheres into the back skin of rats. The results showed that PLLA microspheres retained their shape initially but gradually degraded over time. Histological examinations revealed dense tissue structures around the microspheres, and q-PCR results showed lower p16 mRNA levels at 12 weeks post-injection, indicating a potential deceleration of EpiSC aging. Immunohistochemical staining suggested that PLLA might induce differentiation of EpiSCs.
A New Era in Anti-Aging Treatments
Skin aging involves complex changes in both the epidermis and dermis, leading to thinning, loss of elasticity, wrinkles, and pigmentation. EpiSCs play a crucial role in maintaining skin health and resilience, but their number and functionality decrease with age, leading to diminished regenerative capacity.
PLLA Microspheres: A Game Changer
PLLA microspheres have emerged as a highly effective option for facial rejuvenation. Unlike immediate fillers like hyaluronic acid, PLLA provides gradual and long-lasting effects, making it a preferred choice for many. The study highlights the potential of PLLA to not only stimulate collagen production but also to enhance the proliferation and stemness of EpiSCs, thereby delaying skin aging.
Clinical Implications
The findings of this study offer promising insights into the anti-aging mechanisms of PLLA. The ability of PLLA microspheres to promote cell proliferation, delay senescence, and maintain stemness opens new avenues for developing advanced anti-aging treatments. With further research and clinical trials, PLLA could become a cornerstone in the fight against skin aging, offering a minimally invasive yet highly effective solution for facial rejuvenation.
Conclusion: A Bright Future for Anti-Aging Therapies
This study provides strong evidence of the beneficial effects of PLLA microspheres on EpiSCs and the epidermis. By promoting cell proliferation and delaying aging, PLLA holds the potential to revolutionize anti-aging treatments and facial rejuvenation. As research continues, we can look forward to more effective and long-lasting solutions for maintaining youthful and healthy skin. The future of anti-aging therapies is indeed bright, with PLLA leading the way.
The study findings were published in the peer reviewed journal: Frontiers in Immunology.
https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1394530/full
Thailand Medical News would like to add that PLLA microspheres treatment protocols are currently available at certain aesthetic clinics in Thailand. To know more or to make a booking, contact
MyMediTravel.
https://www.mymeditravel.com/
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Thailand Aesthetics, keep on logging to Thailand Medical News.
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