Study Confirms Gut Microbiota Imbalance (Dysbiosis) Linked To Onset Of Colorectal Cancer
Source: Thailand Medical News Nov 19, 2019 5 years, 1 month, 4 days, 9 hours, 4 minutes ago
A new study by researchers from the gastroenterology team at Henri-Mondor AP-HP Hospital and University Paris-Est Créteil, led by Professor Iradj Sobhani, together with teams from Inserm and the Institut Pasteur Molecular Microbial Pathogenesis Unit (U1202), led by Professor Philippe Sansonetti,holder of the Microbiology and Infectious Diseases Chair at the Collège de France,have demonstrated that an imbalance in the gut microbiota, also known as "
dysbiosis," promotes the onset of
colorectal cancer.
The research teams, operating as the "Oncomix" group since April 2016, demonstrated that transplanting fecal flora from patients with colon
cancer into mice caused lesions and epigenetic changes characteristic of the development of a malignant tumor.
The first research study of its kind, was funded by the French National Cancer Institute and promoted by the Paris Public Hospital Network (AP-HP) as part of a hospital program for clinical cancer research (PHRC-K), led to the development of a non-invasive blood test which identifies the epigenetic phenomenon associated with
dysbiosis. The test was validated in 1,000 individuals. These findings were published in the journal
Proceedings of the National Academy of Sciences (
PNAS).
Spontaneous
colorectal cancer can develop in patients without any known risk factors. It occurs as a result of complex interactions between individuals and their environment. The increasing incidence of this condition reflects negative environmental developments, which can trigger alterations to the genetic and epigenetic DNA of host cells, thereby promoting the onset of sporadic
colorectal cancer.
Many studies have investigated the role of the microbiota as a mediator of these interactions. The team in the Gastroenterology Department at Henri-Mondor AP-HP Hospital and University Paris-Est Créteil, together with a team composed of members from Inserm and the Institut Pasteur Molecular Microbial Pathogenesis Unit (U1202), and the Chair in Microbiology and Infectious Diseases at the Collège de France, demonstrated in mice that the epigenetic mechanism triggered by some bacteria in the microbiota contributes to the onset or promotion of sporadic
colorectal cancer. They subsequently validated their findings in humans.
The 136 animal models in the study were transplanted with either fresh stools from nine patients with sporadic colorectal cancer or fresh stools from nine patients with no colon disorders. The procedure was carried out at Henri-Mondor AP-HP Hospital. The colons of t
he mice were examined 7 and 14 weeks after the human fecal microbiota transplant. The teams particularly investigated the number and development of aberrant crypt foci (or ACF, a type of precancerous lesion), the microbial profile and damage to colonic DNA. They also took into account the animals' food intake, weight and blood indicators.
A major link between fecal
dysbiosis (an imbalance in gut bacteria composition) and the genetic and epigenetic DNA signature in the animals' tissues was identified using statistical tests. Mice who had received fresh stools from patients with sporadic
colorectal cancer developed precancerous lesions known as aberrant crypt foci (ACF) without any significant genetic changes to the colon, but they had a greater number of hypermethylated genes which have been significantly linked to the incidence of ACF in the colonic mucosa.
Professor Iradj Sobhani told
Thailand Medical News in a phone interview, “After analyzing and verifying links between fecal
dysbiosis and DNA anomalies (methylation) in the patients with sporadic
colorectal cancer who took part in the fecal transplants, a pilot study was carried out in humans with the aim of developing a simple, reproducible blood test that can be used for early-stage diagnosis of colorectal tumors in asymptomatic patients. Prospective validation of the test was performed on 1,000 asymptomatic patients who were due to be given a colonoscopy. To identify the bacteria involved, their entire bacterial genome was sequenced. The level of hypermethylation of three genes was defined as a cumulative methylation index (CMI). The patients were classified according to their CMI (positive or negative). An analysis identified a positive CMI as a predictive factor for the onset of sporadic
colorectal cancer.”
This new research shows that the microbiota of subjects with sporadic
colorectal cancer induces precancerous colonic lesions in animals by the hypermethylation of a small number of genes.
The finds also show that the CMI and/or methylating bacteria could be used as diagnostic markers for this type of cancer. These initial findings will need to be evaluated and confirmed in a clinical trial that the team is initiating shortly.
Reference: Iradj Sobhani et al, Colorectal cancer-associated microbiota contributes to oncogenic epigenetic signatures, Proceedings of the National Academy of Sciences (2019). DOI: 10.1073/pnas.1912129116
